Understanding how certain bacterial elements insert DNA in a specific direction.

Structural and evolutionary basis for insertion unidirectionality in RNA-guided DNA transposition systems

['FUNDING_FELLOWSHIP'] · ST. JUDE CHILDREN'S RESEARCH HOSPITAL GRADUATE SCHOOL OF BIOMEDICAL SCIENCES, LLC · NIH-11180723

This study is exploring a special tool called CRISPR-associated transposons that can help scientists insert DNA into specific spots in genes, and it aims to understand how this tool works in one direction, which could lead to better ways to fix genetic disorders.

Quick facts

Phase['FUNDING_FELLOWSHIP']
Study typeNih_funding
SexAll
SponsorST. JUDE CHILDREN'S RESEARCH HOSPITAL GRADUATE SCHOOL OF BIOMEDICAL SCIENCES, LLC (nih funded)
Locations1 site (MEMPHIS, UNITED STATES)
Trial IDNIH-11180723 on ClinicalTrials.gov

What this research studies

This research investigates CRISPR-associated transposons (CAST), which are bacterial genetic elements capable of inserting DNA into specific locations in the genome using a provided RNA sequence. By focusing on the unidirectionality of this insertion process, the research aims to uncover the mechanisms that allow CASTs to insert DNA in a single orientation. The project employs advanced techniques like cryo-electron microscopy to visualize the structural components involved in this process, which could lead to improved genome editing tools for treating genetic disorders.

Who could benefit from this research

Good fit: Ideal candidates for benefiting from this research would be individuals with genetic disorders caused by gene mutations.

Not a fit: Patients with conditions not related to genetic mutations may not receive any benefit from this research.

Why it matters

Potential benefit: If successful, this research could lead to more effective gene therapies for genetic disorders such as cystic fibrosis.

How similar studies have performed: While the specific approach of using CASTs for gene therapy is relatively novel, similar genetic editing techniques have shown promise in other research.

Where this research is happening

MEMPHIS, UNITED STATES

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.

View on NIH RePORTER →

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.