Unbiased amplification of single-stranded DNA oligos for diverse applications
Official title: Unbiased amplification of single stranded DNA oligos across versatile application spaces
Funded at Dnali Data Technologies, INC. · NIH-11409237 · Data updated 2026-11-12
A new platform aims to reliably generate and amplify single-stranded DNA oligos of many lengths for use in genomics, diagnostics, and biotech.
Quick facts
| Grant type | NIH-funded research |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Dnali Data Technologies, INC. NIH-funded |
| Lab location | 1 site (Cary, United States) |
| Project start | September 1, 2026 |
| Project end | August 31, 2027 |
| Funding year | 2026 |
| Award (this year) | $484,634 |
| NIH project number | 1R41HG015248-01 |
| Project ID | NIH-11409237 on NIH RePORTER |
Who could benefit from this research
✓Could help
Not applicable to patient involvement in this stage; the grant targets technology development that could enable future patient-focused tools.
✕Less likely to help
Patients are unlikely to be directly involved or benefit from participation in this project itself.
What this research studies
This project focuses on creating a general method to produce high-fidelity ssDNA across a range of lengths, addressing current biases and inefficiencies. It uses microfluidic laminar flow to improve reagent use and reduce byproducts, then tests various conditions to find optimal amplification protocols for about 200 nt ssDNA. Finally, it maps how well the method works for very short (<30 nt) and very long (>200 nt) ssDNA across different applications. The work blends engineering and biochemistry to unlock faster, cheaper access to ssDNA-dependent technologies.
Why it matters
Potential benefit: If successful, patients could benefit from faster, cheaper development of DNA-based diagnostics, therapeutics, and biotechnologies.
How similar studies have gone: Existing ssDNA amplification methods are biased and limited; this project aims to overcome those gaps with a generalized approach.
Where this research is happening
-
Dnali Data Technologies, INC.
Cary, United States Active
Researchers
- Principal investigator: Tomek, Kyle — Dnali Data Technologies, INC.
- Study coordinator: Tomek, Kyle
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.