Protein-production support for cancer DNA-repair research
Official title: Core 1: Expression and Molecular Biology (EMB)
Funded at University of Calif-Lawrenc Berkeley Lab · NIH-11178324 · Data updated 2026-11-12
This program builds tools and services to help scientists produce high-quality proteins so they can understand how DNA repair fails in cancer.
Quick facts
| Grant type | P01 program project |
|---|---|
| Study type | NIH-funded research |
| Funding institution | University of Calif-Lawrenc Berkeley Lab NIH-funded |
| Lab location | 1 site (Berkeley, United States) |
| Project start | September 27, 2001 |
| Project end | August 31, 2026 |
| Funding year | 2025 |
| Award (this year) | $725,756 |
| NIH project number | 5P01CA092584-25 |
| Project ID | NIH-11178324 on NIH RePORTER |
Who could benefit from this research
✓Could help
This core does not enroll patients—it's a laboratory resource that supports researchers and does not require patient participation.
✕Less likely to help
Patients will not receive direct clinical benefit from the core itself because it focuses on laboratory protein production rather than patient treatment.
What this research studies
This core provides centralized lab services that make and purify proteins researchers need to study DNA repair machinery linked to cancer. It uses modern DNA assembly methods and three expression systems—bacteria, insect cells, and human cells—to produce single- and multi-gene protein complexes. The core incorporates cleavable GFP tags and nanobody-based purification to yield functional, well-behaved proteins and validates protein interactions. It also supports scaled-up production and trains researchers so multiple projects can use the same high-quality materials.
Why it matters
Potential benefit: By speeding and standardizing protein production, this core could help researchers uncover DNA-repair mechanisms that lead to new cancer diagnostics or therapies.
How similar studies have gone: Protein-expression cores and the methods described (Gibson assembly, multi-system expression, GFP-tag purification) are well-established and have supported many successful molecular discoveries in cancer biology.
Where this research is happening
-
University of Calif-Lawrenc Berkeley Lab
Berkeley, United States Active
Researchers
- Principal investigator: Tsai, Miaw-Sheue — University of Calif-Lawrenc Berkeley Lab
- Study coordinator: Tsai, Miaw-Sheue
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.