Oral probiotic therapy to block C. difficile spread

Development of Oral Immunotherapy against Clostridioides difficile Transmission

NIH-funded research University of Maryland Baltimore · NIH-11303466

A friendly probiotic yeast will deliver tiny antibodies in the gut to lower C. difficile spores and help stop the bacteria from spreading among people at risk.

Quick facts

Grant typeR01 grant
Study typeNIH-funded research
Funding institutionUniversity of Maryland Baltimore NIH-funded
Lab location1 site (Baltimore, United States)
Project IDNIH-11303466 on NIH RePORTER

What this research studies

This project engineers a probiotic yeast to release small, single‑domain antibodies that bind a C. difficile surface protein (Cwp84) in the gut. Researchers will screen and optimize bispecific VHH antibodies, produce them in the yeast, and test lead candidates in laboratory and mouse models to see if they reduce bacterial colonization and spore shedding. The goal is to develop an oral immunotherapy that could eventually be tested in people to prevent transmission in hospitals and long‑term care settings.

Who could benefit from this research

Good fit: People at higher risk for C. difficile infection or spread—such as recent antibiotic users, hospitalized patients, or nursing‑home residents—are the eventual target population for this therapy.

Not a fit: Those with unrelated health problems, infections caused by non‑target strains, or who cannot take probiotic yeast (for example, due to specific allergies or contraindications) may not benefit from this approach.

Why it matters

Potential benefit: If successful, this approach could reduce C. difficile colonization and spore shedding, lowering transmission and protecting vulnerable patients from infection.

How similar studies have performed: Related preclinical work, including mouse experiments with engineered yeast, has shown reduced disease severity and spore shedding, but this approach has not yet been tested in humans.

Where this research is happening

Baltimore, United States

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.
Conditions Animal Diseases
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.