New technology to record brain signaling activity at a cellular level
Genomic tools for massively parallel recording of signaling activity at cellular resolution in a brain-wide manner
This study is exploring a new way to use CRISPR technology to track how individual brain cells in zebrafish communicate and develop, which could help us understand important processes that shape the brain over time.
Quick facts
| Grant type | NIH-funded research |
|---|---|
| Study type | NIH-funded research |
| Funding institution | University of Pennsylvania NIH-funded |
| Lab location | 1 site (Philadelphia, United States) |
| Project ID | NIH-10473135 on NIH RePORTER |
What this research studies
This research investigates a novel technology that utilizes CRISPR/Cas tools to record signaling activities in individual brain cells. By employing single-cell RNA sequencing, the study aims to capture dynamic signaling events in the zebrafish brain, focusing on critical pathways like Notch and Fgf. This approach allows for a comprehensive understanding of how these pathways influence cell fate and brain region development over time, moving beyond traditional methods that only analyze bulk cell populations.
Who could benefit from this research
Good fit: Ideal candidates for this research are adults aged 21 and older who may benefit from advancements in understanding brain signaling related to neurological health.
Not a fit: Patients with conditions unrelated to brain signaling or those under 21 years old may not receive any benefit from this research.
Why it matters
Potential benefit: If successful, this research could lead to breakthroughs in understanding brain signaling mechanisms, potentially informing new treatments for neurological conditions.
How similar studies have performed: While the approach of using CRISPR/Cas tools for recording cellular signaling is innovative, similar methodologies have shown promise in other areas of cellular biology, indicating potential for success.
Where this research is happening
Philadelphia, United States
- University of Pennsylvania — Philadelphia, United States (Active)
Researchers
- Principal investigator: Raj, Bushra — University of Pennsylvania
- Study coordinator: Raj, Bushra
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.