New method for analyzing DNA and RNA from the same cell
Official title: Recombinase polymerase amplification to enable same-cell, DNA/RNA multi-omic single cell sequencing
Funded at Sloan-Kettering Inst Can Research · NIH-11004339 · Data updated 2026-11-12
This study is testing a new way to look at both DNA and RNA from individual cells at the same time, which could help us learn more about how genetic differences affect diseases like cancer, ultimately benefiting patients by providing better insights into their health.
Quick facts
| Grant type | R21 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Sloan-Kettering Inst Can Research NIH-funded |
| Lab location | 1 site (New York, United States) |
| Project start | January 1, 2024 |
| Project end | December 31, 2025 |
| Funding year | 2025 |
| Award (this year) | $199,125 |
| NIH project number | 5R21GM152950-02 |
| Project ID | NIH-11004339 on NIH RePORTER |
Who could benefit from this research
✓Could help
Ideal candidates for this research include individuals with genetic disorders or cancers where understanding the genotype-phenotype relationship is crucial.
✕Less likely to help
Patients without genetic conditions or those not requiring detailed genetic analysis may not benefit from this research.
What this research studies
This research focuses on developing a novel technique for single-cell sequencing that allows for the simultaneous analysis of DNA and RNA within the same droplet. By optimizing a process called recombinase polymerase amplification (RPA), the researchers aim to capture both genetic materials without the need for separating them, which is a common limitation in current methods. This approach could enhance our understanding of genetic variations and their implications in diseases like cancer. Patients may benefit from insights gained through this technology, particularly in understanding how genetic factors influence their health.
Why it matters
Potential benefit: If successful, this research could lead to more comprehensive genetic profiling, improving diagnosis and treatment strategies for various conditions.
How similar studies have gone: Other research has shown promise in single-cell sequencing technologies, indicating that this approach could build on existing successes.
Where this research is happening
-
Sloan-Kettering Inst Can Research
New York, United States Active
Researchers
- Principal investigator: Higginson, Daniel — Sloan-Kettering Inst Can Research
- Study coordinator: Higginson, Daniel
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About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.