Investigating RNA modifications in human cells using synthetic mRNA controls
Synthetic mRNA Control Set for Nanopore-Based Pseudouridine Modification Profiling in Human Transcriptomes
This study is looking at how changes to RNA molecules, like adding a special building block called pseudouridine, can affect their stability and function in our cells, which could help improve our understanding of diseases and how cells work.
Quick facts
| Grant type | R01 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Northeastern University NIH-funded |
| Lab location | 1 site (Boston, United States) |
| Project ID | NIH-11015001 on NIH RePORTER |
What this research studies
This research focuses on understanding how specific modifications to RNA molecules, particularly pseudouridine, affect their stability and function in human cells. By creating synthetic mRNA controls that contain these modifications, researchers will utilize advanced nanopore sequencing techniques to accurately detect and quantify pseudouridine levels. This approach aims to enhance the understanding of RNA biology and improve the reliability of RNA sequencing methods, which could lead to better insights into various diseases and cellular processes.
Who could benefit from this research
Good fit: Ideal candidates for this research are individuals with conditions related to RNA processing or diseases where RNA modifications play a significant role.
Not a fit: Patients with conditions unrelated to RNA modifications or those not requiring RNA-based therapies may not benefit from this research.
Why it matters
Potential benefit: If successful, this research could lead to improved RNA-based therapies and diagnostics by providing a more accurate understanding of RNA modifications.
How similar studies have performed: Other research has shown promising results in detecting RNA modifications using nanopore sequencing, indicating that this approach has potential for success.
Where this research is happening
Boston, United States
- Northeastern University — Boston, United States (Active)
Researchers
- Principal investigator: Rouhanifard, Sara Hakim — Northeastern University
- Study coordinator: Rouhanifard, Sara Hakim
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.