Investigating how proteins bind to DNA sequences
Official title: Studying Protein Affinity to DNA by in vitro Transcription and Sequencing (PADIT-seq)
Funded at Brigham and Women's Hospital · NIH-10948634 · Data updated 2026-11-12
This study is looking at how certain proteins interact with DNA to help control gene activity, using a new method that lets researchers see how these proteins bind to many DNA sequences at once, which could help us better understand how genes work and how they might affect our health.
Quick facts
| Grant type | Career grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Brigham and Women's Hospital NIH-funded |
| Lab location | 1 site (Boston, United States) |
| Project start | September 23, 2024 |
| Project end | August 31, 2026 |
| Funding year | 2024 |
| Award (this year) | $136,661 |
| NIH project number | 1K99HG013675-01 |
| Project ID | NIH-10948634 on NIH RePORTER |
Who could benefit from this research
✓Could help
Ideal candidates for this research are individuals with genetic conditions or diseases that involve dysregulation of gene expression.
✕Less likely to help
Patients with conditions unrelated to gene regulation or those not affected by transcription factor activity may not benefit from this research.
What this research studies
This research focuses on understanding how transcription factors (proteins that regulate gene expression) bind to DNA. It employs a novel technique called PADIT-seq, which allows for the testing and quantification of protein-DNA interactions across many DNA sequences simultaneously. By exploring the binding affinities and specificities of these proteins, the research aims to uncover the fundamental principles that govern gene regulation in response to various cellular signals. This could lead to a deeper understanding of genetic regulation and its implications for health and disease.
Why it matters
Potential benefit: If successful, this research could enhance our understanding of gene regulation, potentially leading to new therapeutic strategies for diseases linked to gene expression.
How similar studies have gone: Previous research has shown success in understanding protein-DNA interactions, but the PADIT-seq approach is relatively novel and aims to address existing limitations in the field.
Where this research is happening
-
Brigham and Women's Hospital
Boston, United States Active
Researchers
- Principal investigator: Khetan, Shubham — Brigham and Women's Hospital
- Study coordinator: Khetan, Shubham
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About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.