Investigating how HIV-1 reverse transcriptase changes during drug interactions
"On the Fly" Time Resolved Cryo-EM Studies of Intermediate HIV-1 RT Transition States
['FUNDING_R01'] · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · NIH-10897887
This study is looking at how a key enzyme that helps the HIV virus replicate changes shape when it interacts with certain drugs, which could help us understand why some treatments stop working and lead to better options for people living with HIV.
Quick facts
| Phase | ['FUNDING_R01'] |
|---|---|
| Study type | Nih_funding |
| Sex | All |
| Sponsor | UNIVERSITY OF PITTSBURGH AT PITTSBURGH (nih funded) |
| Locations | 1 site (PITTSBURGH, UNITED STATES) |
| Trial ID | NIH-10897887 on ClinicalTrials.gov |
What this research studies
This research focuses on understanding the dynamic behavior of HIV-1 reverse transcriptase, an enzyme critical for the virus's replication. Using advanced techniques like time-resolved cryo-electron microscopy, the researchers aim to visualize the enzyme's conformational changes as it interacts with nucleosides and their inhibitors. This approach allows for the observation of transient states that occur during the enzyme's activity, which traditional methods cannot capture. By uncovering these mechanisms, the research seeks to provide insights into drug resistance and improve treatment strategies for HIV.
Who could benefit from this research
Good fit: Ideal candidates for this research include individuals living with HIV who are experiencing challenges with current treatments or drug resistance.
Not a fit: Patients who are not infected with HIV or those who have not experienced issues with drug resistance may not benefit from this research.
Why it matters
Potential benefit: If successful, this research could lead to more effective treatments for HIV by enhancing our understanding of drug resistance mechanisms.
How similar studies have performed: Other research has shown success in using advanced imaging techniques to study enzyme dynamics, suggesting that this approach could yield valuable insights into HIV treatment.
Where this research is happening
PITTSBURGH, UNITED STATES
- UNIVERSITY OF PITTSBURGH AT PITTSBURGH — PITTSBURGH, UNITED STATES (ACTIVE)
Researchers
- Principal investigator: CALERO, GUILLERMO ALBERTO — UNIVERSITY OF PITTSBURGH AT PITTSBURGH
- Study coordinator: CALERO, GUILLERMO ALBERTO
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.