Improving the purification process for fluorescent probes used to measure pH levels in organelles
Official title: Preparative binary pump HPLC system with UV and refractive index detector capabilities
Funded at Michigan Technological University · NIH-11034415 · Data updated 2026-11-12
This study is working on creating better tools to measure pH levels inside cells more quickly and accurately, which will help scientists understand how cells work, and it's aimed at researchers who study cellular functions.
Quick facts
| Grant type | R15 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Michigan Technological University NIH-funded |
| Lab location | 1 site (Houghton, United States) |
| Project start | September 18, 2023 |
| Project end | August 31, 2026 |
| Funding year | 2024 |
| Award (this year) | $77,283 |
| NIH project number | 3R15GM152969-01S1 |
| Project ID | NIH-11034415 on NIH RePORTER |
Who could benefit from this research
✓Could help
Ideal candidates for benefiting from this research include individuals with conditions related to cellular dysfunction or metabolic disorders where pH measurement is critical.
✕Less likely to help
Patients with conditions unrelated to cellular pH levels or those not requiring advanced imaging techniques may not benefit from this research.
What this research studies
This research focuses on developing fluorescent probes that can accurately measure pH levels within cellular organelles. The current method of synthesizing these probes is inefficient due to the use of silica gel chromatography, which causes delays. By acquiring a high-performance liquid chromatography (HPLC) system, the research aims to enhance the purification process, making it faster and more effective. This improvement is crucial for producing high-quality probes that can provide better insights into cellular functions.
Why it matters
Potential benefit: If successful, this research could lead to more efficient production of advanced fluorescent probes, improving our understanding of cellular processes.
How similar studies have gone: Similar research approaches using high-performance liquid chromatography have shown promise in improving the efficiency of chemical synthesis and purification processes.
Where this research is happening
-
Michigan Technological University
Houghton, United States Active
Researchers
- Principal investigator: Luck, Rudy Lin — Michigan Technological University
- Study coordinator: Luck, Rudy Lin
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About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.