Improving techniques for determining complex molecular structures using cryo-electron microscopy.
Official title: Tools for Enabling Challenging Structure Determination by Cryo-EM
Funded at Florida State University · NIH-10931729 · Data updated 2026-11-12
This study is working on a new way to look at tiny protein structures that can be tricky to study, which could help scientists better understand diseases related to these proteins, ultimately benefiting patients.
Quick facts
| Grant type | R01 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Florida State University NIH-funded |
| Lab location | 1 site (Tallahassee, United States) |
| Project start | September 19, 2023 |
| Project end | August 31, 2027 |
| Funding year | 2024 |
| Award (this year) | $435,795 |
| NIH project number | 5R01GM148734-02 |
| Project ID | NIH-10931729 on NIH RePORTER |
Who could benefit from this research
✓Could help
Ideal candidates for benefiting from this research include individuals with diseases related to membrane remodeling proteins or other complex molecular structures.
✕Less likely to help
Patients with conditions unrelated to the molecular structures being studied may not receive direct benefits from this research.
What this research studies
This research focuses on enhancing the methods used in cryogenic electron microscopy (cryo-EM) to better analyze complex molecular structures, particularly tubular proteins that are difficult to study. The team has developed a novel technique called reconstruction of average subtracted tubular regions (RASTR), which allows for the breakdown of challenging samples into manageable surfaces for analysis. By addressing issues such as protein denaturation at the air/water interface, this research aims to improve the resolution and reliability of structural determinations. Patients may benefit indirectly through advancements in understanding diseases related to these complex proteins.
Why it matters
Potential benefit: If successful, this research could lead to significant advancements in the understanding and treatment of diseases linked to complex molecular structures.
How similar studies have gone: While the approach of using cryo-EM has shown promise in other studies, the specific technique of RASTR is relatively novel and has not been extensively tested.
Where this research is happening
-
Florida State University
Tallahassee, United States Active
Researchers
- Principal investigator: Stagg, Scott M — Florida State University
- Study coordinator: Stagg, Scott M
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About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.