Improving genetic manipulation techniques in C. elegans without injections
Optimizing RNA import for microinjection-free genetic manipulation in C. elegans
['FUNDING_R21'] · NORTH CAROLINA STATE UNIVERSITY RALEIGH · NIH-11141432
This study is working on a new way to change the genes of tiny worms called C. elegans by using a method that lets them absorb special RNA through their food, making it easier and faster to do genetic research without the need for injections.
Quick facts
| Phase | ['FUNDING_R21'] |
|---|---|
| Study type | Nih_funding |
| Sex | All |
| Sponsor | NORTH CAROLINA STATE UNIVERSITY RALEIGH (nih funded) |
| Locations | 1 site (RALEIGH, UNITED STATES) |
| Trial ID | NIH-11141432 on ClinicalTrials.gov |
What this research studies
This research focuses on enhancing the genetic modification of the model organism C. elegans by developing a method that eliminates the need for microinjections. Instead of injecting nucleic acids into the germline, the project aims to utilize the organism's ability to uptake double-stranded RNA through feeding. By creating RNA carriers that facilitate the efficient delivery of single-guide RNA (sgRNA) throughout the tissues of C. elegans, the researchers hope to streamline the CRISPR/Cas applications for genetic studies. This innovative approach could significantly increase the efficiency of genetic manipulation in high-throughput biology.
Who could benefit from this research
Good fit: Ideal candidates for this research are scientists and researchers working with C. elegans who require efficient genetic manipulation techniques.
Not a fit: Patients who are not involved in genetic research or do not work with C. elegans will likely not benefit from this research.
Why it matters
Potential benefit: If successful, this research could greatly simplify and accelerate genetic studies in C. elegans, leading to faster discoveries in genetics and developmental biology.
How similar studies have performed: While the use of CRISPR/Cas systems is well-established, the specific approach of microinjection-free RNA delivery in C. elegans is novel and has not been extensively tested.
Where this research is happening
RALEIGH, UNITED STATES
- NORTH CAROLINA STATE UNIVERSITY RALEIGH — RALEIGH, UNITED STATES (ACTIVE)
Researchers
- Principal investigator: SAN MIGUEL, ADRIANA — NORTH CAROLINA STATE UNIVERSITY RALEIGH
- Study coordinator: SAN MIGUEL, ADRIANA
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.