Improving genetic manipulation techniques in C. elegans without injections
Official title: Optimizing RNA import for microinjection-free genetic manipulation in C. elegans
Funded at North Carolina State University Raleigh · NIH-11141432 · Data updated 2026-11-12
This study is working on a new way to change the genes of tiny worms called C. elegans by using a method that lets them absorb special RNA through their food, making it easier and faster to do genetic research without the need for injections.
Quick facts
| Grant type | R21 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | North Carolina State University Raleigh NIH-funded |
| Lab location | 1 site (Raleigh, United States) |
| Project start | May 1, 2025 |
| Project end | April 30, 2027 |
| Funding year | 2025 |
| Award (this year) | $398,774 |
| NIH project number | 1R21GM159351-01 |
| Project ID | NIH-11141432 on NIH RePORTER |
Who could benefit from this research
✓Could help
Ideal candidates for this research are scientists and researchers working with C. elegans who require efficient genetic manipulation techniques.
✕Less likely to help
Patients who are not involved in genetic research or do not work with C. elegans will likely not benefit from this research.
What this research studies
This research focuses on enhancing the genetic modification of the model organism C. elegans by developing a method that eliminates the need for microinjections. Instead of injecting nucleic acids into the germline, the project aims to utilize the organism's ability to uptake double-stranded RNA through feeding. By creating RNA carriers that facilitate the efficient delivery of single-guide RNA (sgRNA) throughout the tissues of C. elegans, the researchers hope to streamline the CRISPR/Cas applications for genetic studies. This innovative approach could significantly increase the efficiency of genetic manipulation in high-throughput biology.
Why it matters
Potential benefit: If successful, this research could greatly simplify and accelerate genetic studies in C. elegans, leading to faster discoveries in genetics and developmental biology.
How similar studies have gone: While the use of CRISPR/Cas systems is well-established, the specific approach of microinjection-free RNA delivery in C. elegans is novel and has not been extensively tested.
Where this research is happening
-
North Carolina State University Raleigh
Raleigh, United States Active
Researchers
- Principal investigator: San Miguel, Adriana — North Carolina State University Raleigh
- Study coordinator: San Miguel, Adriana
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About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.