Imaging how enzymes work at the atomic level
NMR crystallography: Imaging active site chemistry and protonation states
This project uses advanced lab imaging and computer models to map every atom in enzymes linked to genetic diseases and antibiotic resistance so scientists can design better treatments.
Quick facts
| Grant type | NIH-funded research |
|---|---|
| Study type | NIH-funded research |
| Funding institution | University of California Riverside NIH-funded |
| Lab location | 1 site (Riverside, United States) |
| Project ID | NIH-11171716 on NIH RePORTER |
What this research studies
You should know that this work is done in the lab on enzyme molecules, not in people. The team combines solid-state NMR (a form of magnetic resonance), X-ray crystallography, and computer chemistry to build detailed atomic models of enzyme active sites. By pinpointing hydrogen atoms and precise charges, they aim to connect enzyme structure to how it works and how drugs might block or fix it. Target enzymes include PLP-dependent enzymes involved in metabolism and beta-lactamases that cause antibiotic resistance.
Who could benefit from this research
Good fit: This project does not enroll patients; it works with purified enzymes, bacterial samples, and computer models in a laboratory setting rather than treating or testing people.
Not a fit: People with immediate treatment needs should not expect direct clinical benefit because this is basic laboratory research that may enable future therapies.
Why it matters
Potential benefit: If successful, the method could help researchers design more effective drugs for metabolic enzyme disorders and new ways to overcome antibiotic resistance.
How similar studies have performed: Related structural methods have supported drug design before, but combining solid-state NMR, crystallography, and computation to map hydrogens in active sites at atomic resolution is still a developing and somewhat novel approach.
Where this research is happening
Riverside, United States
- University of California Riverside — Riverside, United States (Active)
Researchers
- Principal investigator: Mueller, Leonard J — University of California Riverside
- Study coordinator: Mueller, Leonard J
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.