How TDP-43 protein clumps and double-stranded RNA are linked in Alzheimer's and frontotemporal dementia

Defining the pathogenic relationship of TDP-43 inclusions and cytoplasmic double stranded RNA in AD and FTD

NIH-funded research Harvard Medical School · NIH-11297531

This project looks at whether clumps of the protein TDP-43 and abnormal double-stranded RNA appear together in brains of people with Alzheimer's disease or frontotemporal dementia and how that might relate to faster memory loss.

Quick facts

Grant typeR01 grant
Study typeNIH-funded research
Funding institutionHarvard Medical School NIH-funded
Lab location1 site (Boston, United States)
Project IDNIH-11297531 on NIH RePORTER

What this research studies

From a patient's point of view, researchers will study brain tissue donated after death from people who had Alzheimer's disease or frontotemporal dementia. They will use a high-content imaging method called tissue-based cyclic immunofluorescence (t-CyCIF) to map where TDP-43 protein inclusions and cytoplasmic double-stranded RNA (cdsRNA) occur in the same cells. The team will compare many autopsy brains, including cases with the C9ORF72 genetic change, to see whether cdsRNA and TDP-43 inclusions coincide and whether that relates to immune signaling linked to decline. Findings will be compared to earlier laboratory work suggesting cdsRNA activates type I interferon responses.

Who could benefit from this research

Good fit: People with a diagnosis of Alzheimer's disease or frontotemporal dementia who are willing to join a brain donation or tissue-provision program would be appropriate contributors to this work.

Not a fit: People without Alzheimer's or FTD, or those not enrolled in brain donation programs, are unlikely to directly participate or receive immediate benefit from this grant.

Why it matters

Potential benefit: If successful, this work could reveal a biological link that explains why TDP-43 makes dementia progress faster and point to new biomarkers or targets for therapies.

How similar studies have performed: Prior studies have shown TDP-43 is associated with worse cognition and that dsRNA can trigger immune responses in models, but applying multiplexed imaging to map their overlap in many human AD and FTD brains is a newer approach.

Where this research is happening

Boston, United States

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.
Conditions Alzheimer disease dementiaAlzheimer syndromeAlzheimer's DiseaseAlzheimer's disease brainAlzheimer's disease pathology
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.