How slowly‑binding antibiotic molecules change drug activity over time

Mechanism of Slow Onset Enzyme Inhibition and Translation to Time-Dependent Drug Activity

NIH-funded research State University New York Stony Brook · NIH-11167583

This project looks at how the speed that antibiotic drugs stick to and leave their bacterial targets affects how well those drugs work for people with bacterial infections.

Quick facts

Grant typeNIH-funded research
Study typeNIH-funded research
Funding institutionState University New York Stony Brook NIH-funded
Lab location1 site (Stony Brook, United States)
Project IDNIH-11167583 on NIH RePORTER

What this research studies

The team will measure how long candidate antibiotics remain attached to three key bacterial enzymes and how that affects killing bacteria in cell cultures and animal models. They will use structural biology and computer modeling together with enzyme kinetics to build rules linking molecular features to prolonged target binding. Chemists will make new inhibitors designed to stay engaged longer, and a mathematical model will predict time-dependent drug effects including the post-antibiotic effect. Results aim to connect lab measurements to how antibiotics behave in living systems.

Who could benefit from this research

Good fit: Patients with bacterial infections caused by organisms targeted by these classes of antibiotics could be candidates for future clinical testing of drugs developed from this work.

Not a fit: People with viral infections, noninfectious conditions, or bacterial infections not targeted by these enzymes are unlikely to benefit directly from this research.

Why it matters

Potential benefit: If successful, this work could lead to antibiotics designed to remain effective longer in the body, potentially improving treatment of bacterial infections.

How similar studies have performed: Prior studies have shown that longer drug-target residence time can improve activity for some antibiotics, but applying these principles to new bacterial targets is still experimental.

Where this research is happening

Stony Brook, United States

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.
Last reviewed 2026-06-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.