How pachytene piRNAs are made in sperm-forming cells

Genetic and biochemical dissection of mammalian pachytene piRNA biogenesis.

NIH-funded research Michigan State University · NIH-11257666

Researchers will learn how small RNAs called pachytene piRNAs are produced in sperm-forming cells to better understand certain causes of male infertility.

Quick facts

Grant typeNIH-funded research
Study typeNIH-funded research
Funding institutionMichigan State University NIH-funded
Lab location1 site (East Lansing, United States)
Project IDNIH-11257666 on NIH RePORTER

What this research studies

This project uses mouse genetics and laboratory biochemistry to map the steps that create pachytene piRNAs in developing sperm cells. The team will study sequence motifs that guide PIWI proteins, examine how the mitochondrial surface processes long piRNA precursors, and follow how mature PIWI/piRNA complexes leave the processing site to act in the cell. Researchers will combine genetically modified mice with molecular and biochemical experiments to identify key proteins and mechanisms. The goal is to connect these basic molecular steps to spermatogenic failure seen in some cases of human male infertility.

Who could benefit from this research

Good fit: Men with unexplained spermatogenic failure or idiopathic low sperm counts would be the most relevant patient group for translation of these findings.

Not a fit: People with infertility due to female reproductive tract problems, known structural issues, or who need immediate fertility treatment may not directly benefit from this basic research.

Why it matters

Potential benefit: If successful, this work could reveal biological causes of some forms of male infertility and point toward new diagnostic markers or future therapies.

How similar studies have performed: Prior studies in mice and some human genetic reports link defects in the piRNA pathway to male infertility, but the detailed mechanisms of pachytene piRNA production remain largely novel and are still being defined.

Where this research is happening

East Lansing, United States

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.
Conditions Fertility Disorders
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.