How methadone affects the developing human brain

Effect of methadone on the developmental properties of human brain organoids

['FUNDING_R01'] · UNIVERSITY OF CALIFORNIA, SAN DIEGO · NIH-11292421

Looks at whether methadone changes how early human brain cells form and connect in babies exposed before birth.

Quick facts

Phase['FUNDING_R01']
Study typeNih_funding
SexAll
SponsorUNIVERSITY OF CALIFORNIA, SAN DIEGO (nih funded)
Locations1 site (LA JOLLA, UNITED STATES)
Trial IDNIH-11292421 on ClinicalTrials.gov

What this research studies

Researchers grow human brain organoids—small lab-grown models that mimic early fetal brain tissue—to see how methadone exposure changes cell development and nerve connections. They measure cellular and molecular signals and test synaptic activity to find changes in how brain cells communicate. The team builds on preliminary results showing methadone can reduce synaptic transmission in these organoids. All experiments are done in the lab at the university using human-derived cells rather than enrolling patients directly.

Who could benefit from this research

Good fit: This project does not enroll patients directly; its findings are most relevant to pregnant people using methadone and their infants.

Not a fit: People who were not exposed to opioids before birth or those seeking immediate clinical treatment will not directly benefit from this laboratory research.

Why it matters

Potential benefit: If successful, this work could clarify how prenatal methadone exposure affects brain wiring and help guide safer treatment choices or interventions for pregnant people on opioid therapy.

How similar studies have performed: Related laboratory studies and the investigators' preliminary organoid data suggest methadone can alter synaptic signaling, but translating these findings to clinical outcomes is still unproven.

Where this research is happening

LA JOLLA, UNITED STATES

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.

View on NIH RePORTER →

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.