How metabolism affects DNA methylation in EBV-related stomach and throat cancers

Methionine and PI3K Metabolism Drive CIMP in EBV Epithelial Cancers

NIH-funded research Wistar Institute · NIH-11334347

This project looks at whether changing methionine and PI3K-driven metabolism can reverse abnormal DNA methylation in EBV-linked gastric and nasopharyngeal cancers.

Quick facts

Grant typeP01 program project
Study typeNIH-funded research
Funding institutionWistar Institute NIH-funded
Lab location1 site (Philadelphia, United States)
Project IDNIH-11334347 on NIH RePORTER

What this research studies

Researchers are focusing on EBV-positive gastric and nasopharyngeal cancers and analyzing tumor cells' gene activity to find weak points. They use RNA sequencing and CRISPR/Cas9 screens on tumor-derived cells to identify genes that become essential when DNA methylation is lowered with decitabine or when PI3K signaling is blocked with alpelisib. The team is also studying how methionine metabolism supplies the chemical groups that drive the tumor's high DNA methylation. The goal is to find targets and drug combinations that could lead to more precise treatments for EBV-associated epithelial cancers.

Who could benefit from this research

Good fit: People with EBV-positive gastric cancer or EBV-positive nasopharyngeal carcinoma would be the most relevant candidates for therapies arising from this research.

Not a fit: Patients whose tumors are EBV-negative or driven by unrelated pathways are unlikely to benefit from approaches focused on EBV-associated methylation and PI3K biology.

Why it matters

Potential benefit: If successful, this work could point to new targeted treatments that reverse harmful DNA methylation or block PI3K activity to slow or kill EBV-positive stomach and nasopharyngeal cancers.

How similar studies have performed: Hypomethylating drugs and PI3K inhibitors have shown activity in other cancers, but combining methylation reversal with PI3K-targeted approaches specifically for EBV-positive epithelial cancers is a newer strategy with limited prior clinical testing.

Where this research is happening

Philadelphia, United States

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.