How male hormones help form the tubes that become the male internal reproductive organs

Mechanisms of androgen-dependent Wolffian duct differentiation

NIH-funded research University of Wisconsin-Madison · NIH-11318967

This project looks at how male hormones guide the embryonic development of the internal tubes that become the male reproductive tract, which relates to some causes of male infertility.

Quick facts

Grant typeR01 grant
Study typeNIH-funded research
Funding institutionUniversity of Wisconsin-Madison NIH-funded
Lab location1 site (Madison, United States)
Project IDNIH-11318967 on NIH RePORTER

What this research studies

Researchers use genetically modified mice to see how the androgen receptor in supporting tissue controls growth of the Wolffian duct, the embryonic structure that becomes male internal reproductive organs. They compare gene activity and chromatin accessibility using laboratory techniques such as ATAC-seq and RNA analysis to find which signals change in response to male hormones. The team has identified a mesenchymal signal, RSPO3, that appears to activate epithelial WNT signaling needed for proper duct formation and are studying the interaction between mesenchyme and epithelium. Findings are aimed at explaining how disruptions in these signals lead to defective duct development and male infertility.

Who could benefit from this research

Good fit: People affected by congenital malformations of the internal male reproductive tract or men with early-onset unexplained infertility tied to developmental problems could be relevant to the findings of this research.

Not a fit: People whose infertility is caused by later-life issues such as infection, vasectomy, hormonal imbalances unrelated to embryonic duct formation, or female partners will likely not directly benefit from this preclinical work.

Why it matters

Potential benefit: If successful, this work could point to specific molecular signals to target for diagnosing or eventually preventing some forms of congenital male infertility.

How similar studies have performed: Previous animal-model research has shown androgen signaling is essential for Wolffian duct development, but identifying RSPO3 and its role in mesenchyme-to-epithelium signaling is a newer and more specific finding.

Where this research is happening

Madison, United States

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.