How liver cells build and maintain bile channels

Mechanisms of Hepatocyte Polarization and Apical Tube Formation

['FUNDING_R01'] · UNIVERSITY OF PENNSYLVANIA · NIH-11364592

This project aims to understand how liver cells form and keep the tiny bile channels they need to make bile and work properly.

Quick facts

Phase['FUNDING_R01']
Study typeNih_funding
SexAll
SponsorUNIVERSITY OF PENNSYLVANIA (nih funded)
Locations1 site (PHILADELPHIA, UNITED STATES)
Trial IDNIH-11364592 on ClinicalTrials.gov

What this research studies

You can think of liver cells as needing a clear inner plumbing system to move bile; this work uses a special rat liver cell line that makes tube-like bile channels in the lab to model that process. The researchers combine genetic and protein screening with cell biology experiments to study how cell division, cell–cell junctions, the orientation of the dividing machinery, and directed membrane traffic coordinate to form bile canaliculi. They also compare these mechanisms to what happens in mouse livers during development and regeneration to see if the same steps apply in animals. The goal is to map the molecular sequence that turns an initial division-linked canaliculus into a mature tubular bile channel.

Who could benefit from this research

Good fit: People with cholestatic liver disease or those at risk for hepatocellular carcinoma are the kinds of patients who might eventually benefit from advances based on this research.

Not a fit: Patients with conditions unrelated to bile formation or liver polarity are unlikely to receive direct benefit from this specific laboratory-focused work.

Why it matters

Potential benefit: If successful, this work could point to molecular targets to prevent or fix defects in bile formation that cause cholestasis and contribute to some liver cancers.

How similar studies have performed: Prior cell and animal studies have suggested links between cell division, junctional proteins, and canaliculus formation, but the detailed molecular mechanisms remain largely novel and incompletely described.

Where this research is happening

PHILADELPHIA, UNITED STATES

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.

View on NIH RePORTER →

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.