How different C. difficile types affect infection and toxin production

Defining the role of phenotypic heterogeneity in Clostridioides difficile fitness

['FUNDING_R01'] · UNIV OF NORTH CAROLINA CHAPEL HILL · NIH-11233304

This research looks at how different forms of the C. difficile bacterium make toxins and survive in the gut to help people who get C. difficile infections.

Quick facts

Phase['FUNDING_R01']
Study typeNih_funding
SexAll
SponsorUNIV OF NORTH CAROLINA CHAPEL HILL (nih funded)
Locations1 site (CHAPEL HILL, UNITED STATES)
Trial IDNIH-11233304 on ClinicalTrials.gov

What this research studies

Researchers will study how C. difficile switches between forms that have flagella and make toxins and forms that do not, using lab-grown bacteria and mouse infection models. They will examine the genetic 'flg switch' and the signaling molecule c-di-GMP to see how these controls change bacterial behavior. Experiments will measure toxin levels, bacterial motility, and gut inflammation during infection. The team aims to link these bacterial changes to how severe infections become.

Who could benefit from this research

Good fit: People with current or recurrent C. difficile infection, or those willing to donate stool or clinical samples, would be the most relevant participants or future trial candidates.

Not a fit: People without C. difficile infection or with unrelated medical conditions are unlikely to directly benefit from this basic laboratory and animal-focused research.

Why it matters

Potential benefit: If successful, the work could reveal targets to prevent toxin production or reduce severe C. difficile disease.

How similar studies have performed: Previous laboratory and mouse studies have linked flagella and toxin production to C. difficile virulence, but the role of stochastic phase variation via the flg switch in infection outcomes is still being defined.

Where this research is happening

CHAPEL HILL, UNITED STATES

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.

View on NIH RePORTER →

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.