How C. difficile uses DNA methylation to make infectious spores

Epigenetic regulation of sporulation in Clostridioides difficile

['FUNDING_R21'] · TUFTS UNIVERSITY BOSTON · NIH-11266220

Researchers will learn how a unique C. difficile enzyme changes bacterial DNA to produce infectious spores, with the goal of helping people who get C. difficile infections.

Quick facts

Phase['FUNDING_R21']
Study typeNih_funding
SexAll
SponsorTUFTS UNIVERSITY BOSTON (nih funded)
Locations1 site (BOSTON, UNITED STATES)
Trial IDNIH-11266220 on ClinicalTrials.gov

What this research studies

This project looks at a C. difficile enzyme called CamA that chemically modifies the bacterium's DNA and seems to help it form tough, infectious spores. Scientists will use laboratory experiments on C. difficile and related animal models to see how DNA methylation controls spore formation and persistence. By mapping where and how CamA acts, they hope to identify weak points that new drugs could target. The work is done at Tufts and focuses on basic biology that could guide future treatments to prevent recurrence.

Who could benefit from this research

Good fit: People who have had recurrent C. difficile infection or are at high risk for recurrence are the kinds of patients who might benefit from future therapies informed by this work.

Not a fit: People without C. difficile infection or whose illness is caused by other bacteria are unlikely to benefit directly from this basic lab research.

Why it matters

Potential benefit: If successful, the findings could point to new ways to stop C. difficile from making infectious spores and reduce recurrent infections.

How similar studies have performed: Previous lab studies showed CamA is important for spore formation and persistence in mice, but turning that finding into human treatments remains untested.

Where this research is happening

BOSTON, UNITED STATES

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.

View on NIH RePORTER →

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.