How antibiotics that target bacterial DNA enzymes kill infections

Mechanistic Studies of Gyrase/Topoisomerase IV-Targeted Antibacterials

['FUNDING_R01'] · VANDERBILT UNIVERSITY · NIH-11364109

Researchers are finding how common antibiotics called fluoroquinolones damage bacterial DNA machinery to help beat drug-resistant infections like those caused by Acinetobacter.

Quick facts

Phase['FUNDING_R01']
Study typeNih_funding
SexAll
SponsorVANDERBILT UNIVERSITY (nih funded)
Locations1 site (Nashville, UNITED STATES)
Trial IDNIH-11364109 on ClinicalTrials.gov

What this research studies

This project looks closely at two bacterial DNA enzymes, gyrase and topoisomerase IV, which fluoroquinolone antibiotics target to kill bacteria. Scientists will examine how these drugs cause double-stranded DNA breaks and how specific mutations in the enzymes allow bacteria to survive. The team will use biochemical tests and structural analyses of the enzymes and their mutated forms, working with bacterial strains linked to human infections such as Acinetobacter and Bacillus species. The aim is to pinpoint molecular changes that cause resistance so better drugs or strategies can be designed.

Who could benefit from this research

Good fit: People who have had or currently have infections caused by fluoroquinolone-resistant bacteria (for example, resistant Acinetobacter infections) would be most relevant to benefit from the findings.

Not a fit: Patients with viral illnesses or bacterial infections that are not treated with fluoroquinolones are unlikely to see direct benefit from this project in the near term.

Why it matters

Potential benefit: If successful, the work could guide development of new antibiotics or modifications to existing drugs that overcome fluoroquinolone resistance.

How similar studies have performed: Previous structural and genetic research has clarified many aspects of fluoroquinolone action and resistance, so this mechanistic approach builds on established methods to address remaining gaps.

Where this research is happening

Nashville, UNITED STATES

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.

View on NIH RePORTER →

Last reviewed 2026-05-15 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.