HOTTIP RNA and genome organization in acute myeloid leukemia
Role of lncRNA mediated R-loops in CTCF boundary function and AML genome organization
This work looks at whether a noncoding RNA called HOTTIP changes DNA folding in acute myeloid leukemia cells in ways that help the cancer grow.
Quick facts
| Grant type | R01 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Pennsylvania State Univ Hershey Med Ctr NIH-funded |
| Lab location | 1 site (Hershey, United States) |
| Project ID | NIH-11235176 on NIH RePORTER |
What this research studies
Researchers will study how the long noncoding RNA HOTTIP interacts with DNA and the CTCF boundary protein in AML cells to change 3D genome organization. In the lab they will use patient-derived leukemia cells and molecular tools such as RNA-seq, ChIRP-seq, CTCF ChIP-seq, and CRISPR to map HOTTIP binding, detect R-loop structures, and modify HOTTIP or its target sites. The team will watch how these changes alter activity of leukemia-related genes (including HOXA and WNT/β-catenin targets) and behaviors of leukemia cells. This is laboratory-based research that may use human samples but is not a clinical treatment trial.
Who could benefit from this research
Good fit: People with acute myeloid leukemia—especially those whose leukemia has MLL rearrangements or NPM1 mutations—would be the most relevant for sample donation or future trials informed by this work.
Not a fit: Patients without AML or those with unrelated conditions are unlikely to receive direct benefit from this basic laboratory research in the near term.
Why it matters
Potential benefit: If successful, the findings could reveal new molecular mechanisms and targets that eventually lead to therapies that stop AML cells from maintaining their cancerous state.
How similar studies have performed: Previous laboratory studies have shown that lncRNAs can reshape genome organization and influence gene programs, but moving these findings into patient therapies remains largely untested.
Where this research is happening
Hershey, United States
- Pennsylvania State Univ Hershey Med Ctr — Hershey, United States (Active)
Researchers
- Principal investigator: Huang, Suming — Pennsylvania State Univ Hershey Med Ctr
- Study coordinator: Huang, Suming
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.