High-resolution two-photon retinal imaging

High Resolution Functional Imaging of the Retina

NIH-funded research University of Waterloo · NIH-10621149

A near-infrared two-photon imaging approach is being developed to let clinicians see individual retina cells and their chemical activity for people with retinal conditions.

Quick facts

Grant typeR01 grant
Study typeNIH-funded research
Funding institutionUniversity of Waterloo NIH-funded
Lab location1 site (Waterloo, Canada)
Project IDNIH-10621149 on NIH RePORTER

What this research studies

Researchers are building a non-invasive imaging camera that uses near-infrared two-photon light and adaptive optics to make naturally fluorescent molecules in the eye visible at single-cell resolution. The method records both how bright the fluorescence is and how long it lasts after light stimulation to reveal photoreceptor metabolism and neuronal activity. Most work so far is being done in pre-clinical models to refine imaging, timing, and safety before broader human use. The long-term goal is a safe tool to track cellular changes in living eyes over time.

Who could benefit from this research

Good fit: Future human imaging would likely enroll people with photoreceptor-related retinal diseases (for example age-related macular degeneration or inherited retinal degenerations) and healthy volunteers for comparison.

Not a fit: People with dense cataracts or other major media opacities, or those seeking immediate therapeutic benefit rather than diagnostic imaging, would be unlikely to gain direct benefit from this work.

Why it matters

Potential benefit: If successful, this could allow earlier detection of retinal cell damage and non-invasive monitoring of treatment effects at the cellular level.

How similar studies have performed: Two-photon and adaptive-optics retinal imaging have shown promising results in animal studies and early feasibility human work, but remain experimental for routine clinical use.

Where this research is happening

Waterloo, Canada

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.
Last reviewed 2026-06-09 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.