High-resolution protein mapping of single cells with a new tagging array
Advanced Single-Cell Protein Analysis with Multiplex in Situ Tagging Array Technology
This project creates a lab tool that can measure hundreds of proteins inside individual cells to help researchers understand infections and immune-related diseases.
Quick facts
| Grant type | NIH-funded research |
|---|---|
| Study type | NIH-funded research |
| Funding institution | State University New York Stony Brook NIH-funded |
| Lab location | 1 site (Stony Brook, United States) |
| Project ID | NIH-11259535 on NIH RePORTER |
What this research studies
This work builds a very dense microbead 'MIST' array that tags and counts proteins in single cells so tiny protein amounts can be detected. The team has pushed the method to measure hundreds of functional proteins per cell and to map detailed protein patterns on immune cells and small pathogens. They are testing the technology on animal samples, human cells, and microbes to show it can profile both human immune responses and infectious agents. Over time the method could be used on small clinical samples, such as blood, to link cellular protein patterns to outcomes like sepsis.
Who could benefit from this research
Good fit: People with infections, sepsis, immune disorders, or those willing to donate small blood or tissue samples for research would be most relevant to this work.
Not a fit: Patients without immune or infectious conditions, or those unable or unwilling to provide samples, are unlikely to gain direct benefit from this project.
Why it matters
Potential benefit: If successful, the technology could enable earlier, more precise detection of infections and immune responses and guide personalized treatments.
How similar studies have performed: Related single-cell protein methods exist and have shown promise for profiling immune cells, but this approach aims for much higher sensitivity and an unprecedented number of proteins per cell.
Where this research is happening
Stony Brook, United States
- State University New York Stony Brook — Stony Brook, United States (Active)
Researchers
- Principal investigator: Wang, Jun — State University New York Stony Brook
- Study coordinator: Wang, Jun
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.