Fast RNA fluorescence test to find hidden HIV in people on treatment

Sequence-based RNA Fluorescence Assay to Measure Latent HIV Reservoirs

NIH-funded research Jan Biotech, INC. · NIH-11255357

A new lab test aims to detect and measure hidden HIV genetic material in adults whose virus is suppressed by antiretroviral therapy.

Quick facts

Grant typeSbir 2 grant
Study typeNIH-funded research
Funding institutionJan Biotech, INC. NIH-funded
Lab location1 site (Ithaca, United States)
Project IDNIH-11255357 on NIH RePORTER

What this research studies

If you participate, a small blood sample would be tested with a sequence-based RNA fluorescence method that uses chemical amplification and direct detection of cell-associated HIV RNA. The test is designed for people on ART with undetectable viral loads, including those who started treatment very early after infection. Jan Biotech reports the assay can detect HIV RNA in cases where other common tests sometimes fail and that it predicted time to viral rebound in samples from an ACTG cohort. Results may help researchers better identify hidden virus and plan future cure-focused studies.

Who could benefit from this research

Good fit: Adults (21+) living with HIV who have sustained viral suppression on antiretroviral therapy, including those treated during acute/early infection, are the most relevant candidates.

Not a fit: People under 21, those with detectable (unsuppressed) plasma HIV RNA, or those unwilling to provide blood samples are unlikely to benefit directly from this assay.

Why it matters

Potential benefit: If successful, this test could give people and researchers clearer information about hidden HIV levels and help choose candidates for cure trials or treatment-interruption studies.

How similar studies have performed: Common assays (QVOA, qPCR, ddPCR, IPDA, single-copy) can miss reservoir signals in some patients, and this sequence-based fluorescence approach is relatively novel but showed promising predictive results in ACTG A5345 sample analyses.

Where this research is happening

Ithaca, United States

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.
Conditions Acquired Immune Deficiency SyndromeAcquired Immune Deficiency Syndrome VirusAcquired Immunodeficiency SyndromeAcquired Immunodeficiency Syndrome Virus
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.