Fast lab methods to map how proteins fold and move inside cells
High-throughput disulfide and FRET scanning to reveal protein conformational ensembles in vitro and in vivo.
This project builds fast laboratory techniques to map protein shapes and movements inside cells, which could help with infections and diseases caused by misfolded proteins.
Quick facts
| Grant type | NIH-funded research |
|---|---|
| Study type | NIH-funded research |
| Funding institution | State University New York Stony Brook NIH-funded |
| Lab location | 1 site (Stony Brook, United States) |
| Project ID | NIH-11320719 on NIH RePORTER |
What this research studies
Researchers will develop two complementary laboratory techniques: high-throughput disulfide scanning and single-molecule FRET scanning, to measure protein conformations both in test tubes and inside living cells. The team will apply these tools to learn how chaperone proteins work, how bacterial transpeptidases affect antibiotic susceptibility, and how non-native or trapped protein shapes influence cell behavior. The project aims to generate large-scale maps of how proteins change shape across time and many residue pairs. Results will link specific genetic changes to structural and functional outcomes that matter for disease and drug response.
Who could benefit from this research
Good fit: People affected by antibiotic-resistant bacterial infections and patients with diseases involving protein misfolding (for example some neurodegenerative disorders) would be most directly relevant to this work.
Not a fit: Patients with conditions unrelated to infections or protein-folding problems are unlikely to see direct benefits from this project.
Why it matters
Potential benefit: If successful, these tools could reveal new drug targets and help improve antibiotics and treatments for diseases tied to protein misfolding.
How similar studies have performed: Related techniques like single-molecule FRET and disulfide mapping have been used before, but this high-throughput, in-cell combination is novel and less tested.
Where this research is happening
Stony Brook, United States
- State University New York Stony Brook — Stony Brook, United States (Active)
Researchers
- Principal investigator: Serebryany, Evgeny — State University New York Stony Brook
- Study coordinator: Serebryany, Evgeny
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.