Engineered enzymes to break up pneumococcal biofilms and boost protection

Endolysins as tools to eradicate pneumococcal biofilms and development ofprotective immunity

NIH-funded research Univ of Maryland, College Park · NIH-11237108

Using engineered bacteriophage enzymes to break up stubborn Streptococcus pneumoniae (pneumococcus) biofilms and help protect children and older adults.

Quick facts

Grant typeR01 grant
Study typeNIH-funded research
Funding institutionUniv of Maryland, College Park NIH-funded
Lab location1 site (College Park, United States)
Project IDNIH-11237108 on NIH RePORTER

What this research studies

This work develops and tests engineered proteins called endolysins that can cut through the protective biofilm layer pneumococcus makes, allowing bacteria to be killed more easily. In laboratory and animal experiments the team created a stronger endolysin called ClyX-1 that both kills free bacteria and disperses biofilms. They are also studying how breaking up colonization in the nose affects the development of specific antibodies and immune protection. The goal is to combine biofilm disruption with immune responses to reduce carriage and future infections in vulnerable groups.

Who could benefit from this research

Good fit: Ideal candidates for future trials would be people at higher risk for pneumococcal colonization or infection, such as young children and older adults or those with repeated respiratory infections.

Not a fit: People without pneumococcal colonization or those with infections caused by other bacteria are unlikely to benefit from this specific approach.

Why it matters

Potential benefit: If successful, this approach could lead to new treatments that clear biofilms, improve antibiotic effectiveness, and help the body develop protective antibodies against pneumococcus.

How similar studies have performed: Related endolysin therapies have shown promising antibacterial and biofilm-clearing effects in lab and animal studies, but human testing to date has been limited.

Where this research is happening

College Park, United States

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.