Effects of xylazine and fentanyl on breathing and body temperature in rats

Xylazine and fentanyl effects on rat breathing, metabolism, and thermoregulation

NIH-funded research Massachusetts General Hospital · NIH-10950412

This study is looking at how a mix of two drugs, xylazine and fentanyl, affects breathing and body temperature in rats, which could help us understand the risks for people who might overdose on these substances.

Quick facts

Grant typeR21 grant
Study typeNIH-funded research
Funding institutionMassachusetts General Hospital NIH-funded
Lab location1 site (Boston, United States)
Project IDNIH-10950412 on NIH RePORTER

What this research studies

This research investigates how the combination of xylazine, a veterinary sedative, and fentanyl, a synthetic opioid, affects breathing, metabolism, and body temperature in rats. The study aims to understand the toxic effects of these drugs, both individually and together, particularly focusing on their impact on respiratory function and thermoregulation. By measuring various physiological responses, the researchers hope to uncover critical interactions that could inform medical management strategies for overdose victims. The findings may provide insights into how these substances contribute to severe health complications, including respiratory depression and impaired wound healing.

Who could benefit from this research

Good fit: Ideal candidates for benefiting from this research include individuals who are at risk of overdose from opioids or sedatives, particularly those using xylazine and fentanyl.

Not a fit: Patients who do not use opioids or sedatives, or who are not at risk of overdose, may not receive any benefit from this research.

Why it matters

Potential benefit: If successful, this research could lead to improved treatment protocols for individuals affected by xylazine and fentanyl overdoses.

How similar studies have performed: While the specific combination of xylazine and fentanyl has not been extensively studied, research on the effects of opioids and sedatives has shown significant interactions and health risks.

Where this research is happening

Boston, United States

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.
Last reviewed 2026-06-10 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.