Developing new tools to study immune cell gene function
shRNAmir and CRISPR sgRNA Library Construction Core
This study is exploring new ways to understand how genes work in important immune cells, like T cells and B cells, to help us learn more about how our immune system responds to infections, and it's designed for anyone interested in improving immune health.
Quick facts
| Grant type | P01 program project |
|---|---|
| Study type | NIH-funded research |
| Funding institution | University of Florida NIH-funded |
| Lab location | 1 site (Gainesville, United States) |
| Project ID | NIH-10885038 on NIH RePORTER |
What this research studies
This research focuses on creating advanced methods using short hairpin RNAs and CRISPR technology to investigate how genes function in immune cells, specifically CD4 and CD8 T cells and B cells. By developing innovative tools that allow for controlled gene manipulation in these cells, the research aims to enhance our understanding of immune responses. The approach includes generating unmanipulated naive immune cells that can be studied without prior activation, enabling more accurate insights into gene function during immune reactions.
Who could benefit from this research
Good fit: Ideal candidates for this research are individuals with conditions that involve immune system dysfunction, particularly those related to T and B cell activity.
Not a fit: Patients with conditions unrelated to immune cell function or those who do not have active immune disorders may not benefit from this research.
Why it matters
Potential benefit: If successful, this research could lead to improved strategies for manipulating immune responses, which may benefit patients with immune-related conditions.
How similar studies have performed: Other research has shown success with similar CRISPR and RNA interference approaches in studying gene function, indicating a promising avenue for this work.
Where this research is happening
Gainesville, United States
- University of Florida — Gainesville, United States (Active)
Researchers
- Principal investigator: Pipkin, Matthew Eugene — University of Florida
- Study coordinator: Pipkin, Matthew Eugene
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.