Computational design of robust recombinant HRP and scalable HRP reagents
Official title: Computational Protein Design of Robust and Scalable Recombinant HRP and Poly-HRP Reagents
Funded at Monod Bio, INC. · NIH-11316873 · Data updated 2026-11-12
A small SBIR project aims to create a clever, computer-aided design for a new, stable version of horseradish peroxidase (HRP) and related reagents that can be produced reliably in microbes and used in diagnostic tests.
Quick facts
| Grant type | Sbir 1 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Monod Bio, INC. NIH-funded |
| Lab location | 1 site (Seattle, United States) |
| Project start | August 1, 2026 |
| Project end | July 31, 2027 |
| Funding year | 2026 |
| Award (this year) | $330,955 |
| NIH project number | 1R43GM163512-01 |
| Project ID | NIH-11316873 on NIH RePORTER |
Who could benefit from this research
✓Could help
This project is preclinical and material-focused, not a patient study; however, it aims to improve diagnostic assays used on patient samples.
✕Less likely to help
Direct patient participation or results are not part of this phase; relevance comes from improved diagnostics rather than a specific patient intervention.
What this research studies
This project uses advanced computer design to create a recombinant HRP enzyme that is stable, active, and easy to manufacture in microbial systems. It also explores assembling HRP into nanoparticle reagents to improve performance and consistency across batches. The goal is a reproducible, scalable HRP solution that works in common diagnostic tools like ELISA and immunohistochemistry. By addressing glycosylation, disulfide bonding, and cofactor incorporation, the work seeks to overcome past manufacturing hurdles. The SBIR focus is on delivering a practical, manufacturable reagent to improve test reliability in clinical and research settings.
Why it matters
Potential benefit: If successful, patients could benefit from more reliable diagnostic tests with consistent performance across lots.
How similar studies have gone: Past attempts to create recombinant HRP have faced consistency issues, making this approach novel but with a foundation of prior work in enzyme design and HRP-based assays.
Where this research is happening
-
Monod Bio, INC.
Seattle, United States Active
Researchers
- Principal investigator: Quijano Rubio, Alfredo — Monod Bio, INC.
- Study coordinator: Quijano Rubio, Alfredo
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.