Cis-acting preferences in LINE-1 replication and ribonucleoprotein assembly
Official title: Mechanisms and dynamics of cis preference in LINE-1 replication
Funded at Rockefeller University · NIH-11429361 · Data updated 2026-11-12
This grant explores how LINE-1 elements copy themselves in human cells by using ribonucleoprotein complexes that may be guided by translation in a way similar to some viruses.
Quick facts
| Grant type | R01 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Rockefeller University NIH-funded |
| Lab location | 1 site (New York, United States) |
| Project start | July 1, 2026 |
| Project end | June 30, 2030 |
| Funding year | 2026 |
| Award (this year) | $1,356,000 |
| NIH project number | 1R01GM166058-01 |
| Project ID | NIH-11429361 on NIH RePORTER |
Who could benefit from this research
✓Could help
Not a patient-facing study; the work is basic science using cell systems and molecular assays.
✕Less likely to help
Clinical participants are not enrolled in this project.
What this research studies
The project aims to understand how LINE-1 elements copy their RNA into DNA by forming a ribonucleoprotein complex with ORF1p and ORF2p and how cis preference is enforced during this process. Researchers will examine co-translational and co-assembly mechanisms that may localize replication to the RNA that encodes it, comparing these processes to viral strategies. The work involves detailed molecular biology approaches to test how translation and protein-RNA interactions shape LINE-1 replication dynamics in human cells. Outcomes could reveal new targets for limiting harmful LINE-1 activity tied to disease, while enriching our understanding of genome biology. The study is led by a senior investigator at Rockefeller University and will be conducted over four years with support from NIH funding.
Why it matters
Potential benefit: A better understanding of LINE-1 biology could lead to insights about genome stability and potential therapeutic strategies for diseases linked to retrotransposon activity.
How similar studies have gone: The approach builds on established knowledge of LINE-1 biology and viral-like cis-activity patterns, with potential for novel mechanistic insights.
Where this research is happening
-
Rockefeller University
New York, United States Active
Researchers
- Principal investigator: Schneider, William Matthew — Rockefeller University
- Study coordinator: Schneider, William Matthew
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About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page.