Changing a bacterial lipid to understand how E. coli builds its protective outer coating

Manipulating undecaprenyl phosphate levels to decipher mechanisms of competing cell envelope assembly pathways in Escherichia coli

NIH-funded research Univ of Arkansas for Med Scis · NIH-11319854

Researchers will change levels of a bacterial lipid called undecaprenyl phosphate in E. coli to learn how the microbes build the protective layers that antibiotics target, aiming to guide better treatments for infections.

Quick facts

Grant typeNIH-funded research
Study typeNIH-funded research
Funding institutionUniv of Arkansas for Med Scis NIH-funded
Lab location1 site (Little Rock, United States)
Project IDNIH-11319854 on NIH RePORTER

What this research studies

As someone following infection research, this project focuses on a lipid called undecaprenyl phosphate (Und-P) that bacteria use to assemble surface sugars and the cell wall. Scientists will use genetic and chemical tools to raise or lower Und-P levels and observe how those changes alter construction of the inner membrane, peptidoglycan cell wall, and outer membrane in E. coli. They will also manipulate the enzyme pathways that both make and use Und-P to see how these pathways compete for the shared lipid carrier. The team will map which processes depend on Und-P and look for vulnerabilities that new antibiotics or vaccines might exploit.

Who could benefit from this research

Good fit: Patients with E. coli infections, especially recurrent or antibiotic-resistant cases, are the group most likely to benefit from therapies informed by this research.

Not a fit: This lab-based basic research will not provide immediate treatment or direct clinical benefit to patients seeking urgent care.

Why it matters

Potential benefit: If successful, this work could identify new targets on the bacterial cell envelope that lead to better antibiotics or vaccine components against E. coli and related pathogens.

How similar studies have performed: Previous studies show that reducing Und-P can weaken bacteria, but deliberately increasing Und-P and mapping competition among Und-P-using pathways is largely untested and represents a novel approach.

Where this research is happening

Little Rock, United States

Researchers

About this research

  1. This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
  2. Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
  3. For full project details, budget, and progress reports, visit the official NIH RePORTER page below.
Last reviewed 2026-06-13 by the Find a Trial editorial team. Information on this page is for educational purposes and is not medical advice. Always consult qualified healthcare professionals about clinical trial participation.