Better testing to find antibiotic resistance genes in bacteria
Triple-enriched metagenomics for robust resistome analysis
A new lab method to find and sequence antibiotic resistance genes in bacteria from clinical and environmental samples.
Quick facts
| Grant type | R01 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | University of Minnesota NIH-funded |
| Lab location | 1 site (Minneapolis, United States) |
| Project ID | NIH-11394275 on NIH RePORTER |
What this research studies
This project will create a laboratory workflow that enriches and sequences DNA to better detect antibiotic resistance genes across whole microbial communities like the gut or wound microbiome. It combines improved probe-based capture, real-time enrichment during long-read sequencing, and a 'triple-enrichment' approach to increase how many resistance genes are found and how accurately they are placed in their microbial context. Researchers will test and refine the method using many types of samples with different resistance gene mixtures. The goal is faster, lower-cost, and more complete resistance profiles that could support care and public health tracking.
Who could benefit from this research
Good fit: Ideal contributors would include people with recent bacterial infections, recent antibiotic use, or those willing to provide samples such as stool, swabs, or clinical specimens for sequencing.
Not a fit: People seeking direct treatment from this project are unlikely to benefit immediately, since it focuses on laboratory method development rather than providing clinical therapy.
Why it matters
Potential benefit: If successful, this could help clinicians and public health teams detect and track antibiotic resistance more quickly and accurately, informing better treatment and prevention.
How similar studies have performed: Related probe-capture and long-read sequencing methods have shown promise for detecting resistance genes, but the proposed triple-enrichment approach is new and not yet widely validated.
Where this research is happening
Minneapolis, United States
- University of Minnesota — Minneapolis, United States (Active)
Researchers
- Principal investigator: Noyes, Noelle — University of Minnesota
- Study coordinator: Noyes, Noelle
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.