Analyzing RNA modifications in brain tissues at a single-cell level
Single-cell in situ analysis of RNA modifications in intact tissues
This study is looking at how tiny chemical changes in RNA, which are important for how our genes work, can affect health and disease, and it will use mouse brain tissue to learn more about these changes in different types of cells.
Quick facts
| Grant type | NIH-funded research |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Broad Institute, INC. NIH-funded |
| Lab location | 1 site (Cambridge, United States) |
| Project ID | NIH-11014638 on NIH RePORTER |
What this research studies
This research focuses on understanding the complex chemical modifications of RNA, known as the epitranscriptome, which play a crucial role in gene expression and are linked to various human diseases. By developing advanced tools for three-dimensional in situ sequencing, the project aims to investigate how these RNA modifications affect gene regulation in different cell types within intact biological tissues. The study will specifically utilize mouse brain tissue to explore the N6-methyladenosine (m6A) modification pathway, providing insights into the spatial and cellular dynamics of RNA modifications.
Who could benefit from this research
Good fit: Ideal candidates for this research are individuals with conditions linked to RNA modification dysregulation, such as certain neurological disorders.
Not a fit: Patients with conditions unrelated to RNA modifications or those not involving gene expression regulation may not benefit from this research.
Why it matters
Potential benefit: If successful, this research could lead to a better understanding of gene regulation mechanisms, potentially informing new treatments for diseases associated with RNA modification alterations.
How similar studies have performed: While the approach of analyzing RNA modifications in situ is innovative, similar methodologies have shown promise in other areas of molecular biology, suggesting potential for success.
Where this research is happening
Cambridge, United States
- Broad Institute, INC. — Cambridge, United States (Active)
Researchers
- Principal investigator: Wang, Xiao — Broad Institute, INC.
- Study coordinator: Wang, Xiao
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.