A new method to analyze gene expression in cancer cells using CRISPR technology
Stitch-seq for genome-wide pooled genomic screening with RNA-seq readout
This study is testing a new method to better understand how genes in cancer cells work and respond to treatments, which could help find new ways to fight cancer and improve care for patients like you.
Quick facts
| Grant type | R21 grant |
|---|---|
| Study type | NIH-funded research |
| Funding institution | Broad Institute, INC. NIH-funded |
| Lab location | 1 site (Cambridge, United States) |
| Project ID | NIH-10792615 on NIH RePORTER |
What this research studies
This research focuses on developing a novel technique called Stitch-seq, which allows for the analysis of gene expression in cancer cells through pooled CRISPR screening. By linking CRISPR guide RNA sequences to targeted mRNA sequences, this method enables researchers to identify how different genes influence cell behavior and response to treatments. The approach is designed to be cost-effective and efficient, making it feasible to conduct large-scale genomic screenings that can provide insights into cancer biology. Patients may benefit from the findings as they could lead to improved understanding of cancer mechanisms and potential new therapeutic targets.
Who could benefit from this research
Good fit: Ideal candidates for this research are individuals diagnosed with various types of cancer who may benefit from new therapeutic strategies.
Not a fit: Patients with non-malignant conditions or those not affected by cancer may not receive any benefit from this research.
Why it matters
Potential benefit: If successful, this research could lead to breakthroughs in understanding cancer biology and developing more effective treatments.
How similar studies have performed: Other research has shown success with similar CRISPR screening approaches, indicating a promising avenue for cancer research.
Where this research is happening
Cambridge, United States
- Broad Institute, INC. — Cambridge, United States (Active)
Researchers
- Principal investigator: Blainey, Paul Clark — Broad Institute, INC.
- Study coordinator: Blainey, Paul Clark
About this research
- This is an active NIH-funded research project — typically early-stage science, not a clinical trial accepting patient enrollment.
- Some NIH-funded labs run parallel clinical studies or seek volunteers for related work. To check, contact the principal investigator or institution listed above.
- For full project details, budget, and progress reports, visit the official NIH RePORTER page below.